AQUEOUS EXTRACTION OF ANTIOXIDANT KAPPA-CARRAGEENAN FROM Hypnea musciformis (RHODOPHYTA) MARICULTURE
DOI:
https://doi.org/10.18817/repesca.v16i3.3696Resumo
Mariculture of Hypnea musciformis (Rhodophyta) on the northeast coast of Brazil has been an alternative in bioactives sulfated polysaccharides (HmSPs). This study aimed to extract in hot-water HmSPs to quantification and determination of their structural and antioxidant properties. After cold-water pretreatment (1.5%, w v-1), algal residue was reextracted in water (80°C, 4 h) and HmSPs characterized by agarose/polyacrylamide gels electrophoreses vs. standard glycosaminoglycans stained with toluidine blue/Stains-All; and then by infrared analyzed. HmSPs were in vitro tested for antioxidant effects by DPPH, total antioxidant capacity (TAC) and ferrous ion chelating methods against BHT, ascorbic acid and EDTA, respectively. Refined aqueous extraction (20.00 ± 3.25%, w w-1) revealed kappa-carrageenan made of a polydisperse charge system (>100 kDa) with structural peculiarities, but without presenting chemical degradation. HmSPs had antioxidant effects by all the tests, but revealing prepondetant role by TAC initiation phase than in propagation one with less efficacy than respective synthetics. Therefore, mariculture of H. musciformis may be a valuable alternative to antioxidant SPs obtaining.







